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mouse cell depletion kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec mouse cell depletion kit
    Mouse Cell Depletion Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/depletion/Mouse+Cell+Depletion+Kit/pmc13637652-56-20-24
    Average 96 stars, based on 379 article reviews
    mouse cell depletion kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics.
    Article Snippet: .. Cells from the B-cell lineage (including IgGsecreting cells) were enriched using the Pan B-cell isolation kit II (Miltenyi Biotec) as described2 and were further enriched by depletion with anti-IgM (Miltenyi Biotec, 130-095-904) and anti-IgD (Miltenyi Biotec, 130-101-899) antibodies. .. Cells from the B-cell lineage (including IgGsecreting cells) were enriched using the Pan B-cell isolation kit II (Miltenyi Biotec) as described2 and were further enriched by depletion with anti-IgM (Miltenyi Biotec, 130-095-904) and anti-IgD (Miltenyi Biotec, 130-101-899) antibodies.

    Article Title: Systematic design of combination therapy by targeting master regulators of coexisting diffuse midline glioma cell states.
    Article Snippet: .. Pellets were resuspended in isolation media (1 ml) and incubated with myelin removal beads (100 μl, 15 min; Miltenyi Biotec, 130-096-433), followed by depletion using LS-positive separation columns (Miltenyi Biotec, 130-042-401). ..

    Incubation:

    Article Title: Failure of Alzheimer’s Mice Brain Resident Neural Precursor Cells in Supporting Microglia-Mediated Amyloid β Clearance
    Article Snippet: The SVZ extracts from each mouse group were pooled and dissociated to a single-cell suspension using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) according to the manufacturer’s protocol. .. For myelin removal, the cells were incubated with Myelin Removal Beads II (Miltenyi Biotec, 130-096-733) according to the manufacturer’s protocol, followed by depletion with LS columns (Miltenyi Biotec, 130-042-401), resulting in a myelin-free single-cell suspension. .. The cells were counted and plated in a 24-well plate (300,000 cells per well) in a B27 (Gibco, Waltham, MA, USA, 12587010) supplemented DMEM/F12-GlutaMAX (Gibco) [ ] medium.

    Article Title: Systematic design of combination therapy by targeting master regulators of coexisting diffuse midline glioma cell states.
    Article Snippet: .. Pellets were resuspended in isolation media (1 ml) and incubated with myelin removal beads (100 μl, 15 min; Miltenyi Biotec, 130-096-433), followed by depletion using LS-positive separation columns (Miltenyi Biotec, 130-042-401). ..

    Single Cell:

    Article Title: Failure of Alzheimer’s Mice Brain Resident Neural Precursor Cells in Supporting Microglia-Mediated Amyloid β Clearance
    Article Snippet: The SVZ extracts from each mouse group were pooled and dissociated to a single-cell suspension using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) according to the manufacturer’s protocol. .. For myelin removal, the cells were incubated with Myelin Removal Beads II (Miltenyi Biotec, 130-096-733) according to the manufacturer’s protocol, followed by depletion with LS columns (Miltenyi Biotec, 130-042-401), resulting in a myelin-free single-cell suspension. .. The cells were counted and plated in a 24-well plate (300,000 cells per well) in a B27 (Gibco, Waltham, MA, USA, 12587010) supplemented DMEM/F12-GlutaMAX (Gibco) [ ] medium.

    Article Title: Failure of Alzheimer’s Mice Brain Resident Neural Precursor Cells in Supporting Microglia-Mediated Amyloid β Clearance
    Article Snippet: Brain tissue was dissociated to a single-cell suspension using a neural tissue dissociation kit (P) (130-092-628, Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol, followed by Myelin removal using Percoll (GE17-0891-01, Sigma-Aldrich-Merck, St. Louis, MO, USA). .. Sorting for CD11b+ cells was achieved by magnetic CD11b (Microglia) MicroBeads (130-093-634, Miltenyi Biotec), according to the manufacturer’s protocol followed by depletion with MS columns (130-042-201, Miltenyi Biotec), resulting in CD11b+ single-cell suspension. .. Cells were counted and plated on Poly-D-Lysine (10 μg/1 mL, Sigma-Aldrich-Merck) coating of either 24-well plate cover slips (150,000 per well), 35 mm plate (Ibidi, Gräfelfing, Germany, 81158, 850,000 cells, for a time-lapse experiment, described below) or 6-well plate cover slips (1 million per well, for RNA extraction, described below).

    Suspension:

    Article Title: Failure of Alzheimer’s Mice Brain Resident Neural Precursor Cells in Supporting Microglia-Mediated Amyloid β Clearance
    Article Snippet: The SVZ extracts from each mouse group were pooled and dissociated to a single-cell suspension using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) according to the manufacturer’s protocol. .. For myelin removal, the cells were incubated with Myelin Removal Beads II (Miltenyi Biotec, 130-096-733) according to the manufacturer’s protocol, followed by depletion with LS columns (Miltenyi Biotec, 130-042-401), resulting in a myelin-free single-cell suspension. .. The cells were counted and plated in a 24-well plate (300,000 cells per well) in a B27 (Gibco, Waltham, MA, USA, 12587010) supplemented DMEM/F12-GlutaMAX (Gibco) [ ] medium.

    Article Title: Failure of Alzheimer’s Mice Brain Resident Neural Precursor Cells in Supporting Microglia-Mediated Amyloid β Clearance
    Article Snippet: Brain tissue was dissociated to a single-cell suspension using a neural tissue dissociation kit (P) (130-092-628, Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol, followed by Myelin removal using Percoll (GE17-0891-01, Sigma-Aldrich-Merck, St. Louis, MO, USA). .. Sorting for CD11b+ cells was achieved by magnetic CD11b (Microglia) MicroBeads (130-093-634, Miltenyi Biotec), according to the manufacturer’s protocol followed by depletion with MS columns (130-042-201, Miltenyi Biotec), resulting in CD11b+ single-cell suspension. .. Cells were counted and plated on Poly-D-Lysine (10 μg/1 mL, Sigma-Aldrich-Merck) coating of either 24-well plate cover slips (150,000 per well), 35 mm plate (Ibidi, Gräfelfing, Germany, 81158, 850,000 cells, for a time-lapse experiment, described below) or 6-well plate cover slips (1 million per well, for RNA extraction, described below).

    Selection:

    Article Title: Tumor-associated myeloid cells support murine T-ALL in the central nervous system via integrin signaling
    Article Snippet: .. Cells from the leukemic CNS and spleen were split into 2 groups: one group was enriched for T-ALL by incubating with biotinylated antibodies against CD11c, CD11b, F4/80, and I-A/I-E, followed by depletion with streptavidin beads (Mojosort; BioLegend); and the other group was enriched for myeloid cells by incubating with biotinylated antibodies against CD11c followed by selection with streptavidin beads (MACS; Miltenyi). ..

    Magnetic Cell Separation:

    Article Title: Tumor-associated myeloid cells support murine T-ALL in the central nervous system via integrin signaling
    Article Snippet: .. Cells from the leukemic CNS and spleen were split into 2 groups: one group was enriched for T-ALL by incubating with biotinylated antibodies against CD11c, CD11b, F4/80, and I-A/I-E, followed by depletion with streptavidin beads (Mojosort; BioLegend); and the other group was enriched for myeloid cells by incubating with biotinylated antibodies against CD11c followed by selection with streptavidin beads (MACS; Miltenyi). ..

    Article Title: Changes in protein fluxes in skeletal muscle during sequential stages of muscle regeneration after acute injury in male mice
    Article Snippet: .. Single muscle fibers with attached fiber-associated cells were then depleted of infiltrating CD45+ cells by undergoing magnetic assisted cell sorting (MACS) mediated selective depletion, according to manufacturer's instructions (Miltenyi Biotec #130-052-301), before being processed for in-solution digestion. ..

    FACS:

    Article Title: Changes in protein fluxes in skeletal muscle during sequential stages of muscle regeneration after acute injury in male mice
    Article Snippet: .. Single muscle fibers with attached fiber-associated cells were then depleted of infiltrating CD45+ cells by undergoing magnetic assisted cell sorting (MACS) mediated selective depletion, according to manufacturer's instructions (Miltenyi Biotec #130-052-301), before being processed for in-solution digestion. ..



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